We used alloxan to induce diabetes in rats and rabbits for screening the antidiabetic activity of test compounds. However, in absence of aggressive stimuli such as ligatures, the influence of diabetes on. Dec 18, 2007 alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter. This study aimed to determine the protective effect of curcumin on streptozotocin stz induced oxidative stress in various tissues of albino wister rats. Alloxan induced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. Antihyperglycaemic activity of cassia kleinii leaf extract in glucose fed normal rats and alloxan induced diabetic rats v babu, t gangadevi, a subramoniam. Early renal histological changes in alloxaninduced diabetic rats mohsen pourghasem 1, ebrahim nasiri 2, hamid shafi 3 1. Research article alloxan induced diabetes mellitus in. Cellular and molecular biology research center cmbrc, babol university of medical sciences, babol, iran. Morin treatment attenuated hyperalgesia and analgesia p diabetic rats at both doses significantly decreased the levels of cytokines p diabetic neuropathy induced by injection with streptozotocin stz. A closed fracture was performed on the left tibia of 3monthold wistar rats weighing 250 to 350 g that were either healthy n 24 or made diabetic with alloxan. A crude extract of carica papaya seeds was prepared in boiling water and the aqueous extract was dried.
In the present study, we report the effects of induced diabetes and its treatment with or without oral hypoglycaemic drugs on the morphology and. We used alloxan to induce diabetes in rats and rabbits for screening the antidiabetic. The mechanisms of alloxan and streptozotocininduced. This study is aimed to evaluate the antidiabetic activity of ethanolic extracts of s. Diabetes was induced by streptozotocin 40 mgkg in female wistar rats. The effects of ethanol extracts on glucose tolerance tests were significant. Osim2 1department of physiology, faculty of basic medical sciences, college of health sciences, university of uyo, uyo, akwa ibom state. Antioxidant effect of curcumin extracts in induced diabetic. At a dose of 100 mgkg, 200mgkg the extract was given to male sprague dawley rats for 14 days to evaluate the anti hyperglycemic and anti hyperlipidaemic activity in streptozotocin nicotinamide induced diabetic rats. The rs504393 influences the level of nociceptive factors. The endpoint measures are blood glucose levels, body weight, and a standard behavioral measure of pain threshold. Experimental models play an important role in understanding such a disease, which is treatable only. Optimization of alloxan dose is essential to induce stable. Alloxan induced insulin release is, however, of short duration and is followed by complete suppression of the islet response to glucose, even when high concentrations 16.
Adeniyi paulina oludoyin 1, 2, sanusi rasaki adegoke 1. In the majority of experiments, na is given to rats 15 min before stz to induce niddm. Single dose study after single dose of the mesc 200 or 400 mgkg, p. Alloxaninduced diabetes, a common model for evaluating. The clinical course of the diabetes mortality, hyperglycemia, weight loss, polydipsia, hyperphagia, polyuria, glycosuria and diabetic indices was recorded for six weeks before the animals were sacrificed for volumetric quantitation of the pancreatic islets. Nitric oxide and inflammatory cytokine productions in diabetic rats supplemented with goat milk and soy milk kefir.
This study was conducted to investigate the effect of nicotinamide on experimentally induced iddm. Obri department of anatomy, faculty of basic medical sciences, university of calabar, calabar, nigeria. Mechanisms of inhibited lipolytic response to isoproterenol. The present study was carried out to evaluate the antidiabetic activity of vinca rosea methanolic whole plant extracts in alloxan induced diabetic rats for 14 days. The current study sought to investigate the capacities of single and combinatorial herbal formulations of leaf extracts of acanthus montanus, asystasia gangetica, emilia coccinea, and hibiscus rosasinensis to reverse hyperglycemia and dyslipidemia in alloxan induced diabetic male rats. Diabetes was induced in rats with equal molar dosages of either streptozotocin or alloxan. Blood glucose level and lipid profile of alloxaninduced.
Functional consequences after buserelininduced enteric. Fauna 3 kampus ugm, bulaksumur, yogyakarta 55281, indonesia. Variables in alloxan induced and fructose induced diabetic rats adesoji a fasanmade and oluwakemi t alabi departments of physiology and medicine college of medicine, university of ibadan ibadan, nigeria abstract honey contains a high concentration of fructose, a monosaccharide, capable of raising blood sugar level after oral ingestion. Early renal histological changes in alloxaninduced. Rathore1, raghuveer choudhary2, rajesh chaudhary3 1. Treatment of alloxaninduced diabetic rats with metformin or. Streptozotocin induced experimental diabetes male wistar rats pdf. The mechanism of alloxan and streptozotocin action in b cells. Effect of ginger zingiber officinale extracts on blood.
Nitric oxide and inflammatory cytokine productions in. Albino wistar rats with streptozotocin induced diabetes were divided into. The use of experimentally induced diabetes in rats remains as one of the easiest and convenient methods for screening of new drugs. Streptozotocin induced diabetes in wistar albino rats is neither type i nor type ii. In alloxan induced diabetic rats by oral administration of extract 100mgkg body weight. In the presence of intracellular thiols, especially glutathione, alloxan generates reactive oxygen species ros in a cyclic redox reaction with its reduction product, dialuric acid.
These findings suggest that morphologic aberrations are provoked by untreated diabetes mellitus, even in the absence of oxidative stress. This study aimed to determine the protective effect of curcumin on streptozotocin stzinduced oxidative stress in various tissues of albino wister rats. It has been successfully induced in a variety of animal species. Evaluation of antidiabetic activity of ginkgo biloba in streptozotocin. Enhancement of bilirubin excretion in alloxan induced diabetic rats treated with crude aloe vera gel u.
Department of anatomical sciences, gilan university of medical sciences, rasht, iran. The mechanisms of alloxan and streptozotocininduced diabetes. Thirty spraque dawley rats were divided into 3 groups. We read with interest the article titled anomalies in alloxaninduced diabetic model. Periodontal disease in diabetic patients presents higher severity and prevalence. Antidiabetic activity of mediumpolar extract from the. Healthy rats were confirmed as diabetic after treatment with alloxan. Autoxidation of dialuric acid generates superoxide radicals. This study evaluated the longterm effects of alloxaninduced diabetes in rat liver. The protective effect of nigella sativa against cisplatin.
Comparison of streptozotocin and alloxaninduced diabetes in. Alloxan induced diabetic rats treated with ethanol extract of smilax chinensis l. Bauhinia holophylla leaves treatment on the streptozotocininduced diabetic rats. Antidiabetic effect of morinda lucida stem bark extracts on alloxaninduced diabetic rats.
Intravenous injection of alloxan into rabbits, rats, dogs, monkeys, etc. Anti diabetic activity of ethanolic extract of aconitum ferox. Antidiabetic effect of sida cordata in alloxan induced diabetic rats. Experimental model of induction of diabetes mellitus in rats. The 4th and 5th diabetic groups were given glibenclamide 5. The protective effect of nigella sativa against cisplatininduced nephrotoxicity in rats sara hosseinian1, abolfazl 2khajavi rad, mousaalreza hadjzadeh3, nema mohamadian roshan4, shahrzad havakhah1, somayeh shafiee1 1department of physiology, school of medicine, mashhad university of medical sciences, mashhad, iran. Early renal histological changes in alloxaninduced diabetic.
Diabetes induction and animals selection were followed as described in glucose tolerance test in diabetic. Which type of diabetes will be induced by streptozotocin in. Stz and alloxan induced diabetic mice scientist solutions. The methanolic whole plant extract at high dose 500 mgkg exhibited significant antihyperglycemic activity than whole plant extract at low dose 300 mgkg in diabetic rats. A streptozotocin stz induced diabetes in animal model mimics, in some aspects, recent onset iddm. Alloxaninduced diabetes delays repair in a rat model of.
Anti diabetic effect of cherries in alloxan induced diabetic rats. Hence it is vital to establish a dosage of drugs used in induction of experimental diabetes so as to develop stable diabetic characteristics with minimal drug toxicity. Biochemical study on the hypoglycemic effects of onion and. The diabetic rats were treated with moringa extract as described in section 2. It is used as a cleansing and purifying agent and yet can be considered as a bodybuilder because of its nutritional value. Effect of nicotinamide on experimental induced diabetes. At a dose of 100 mgkg, 200mgkg the extract was given to male sprague dawley rats for 14 days to evaluate the anti hyperglycemic and anti hyperlipidaemic activity in streptozotocin.
Alloxan diabetes and streptozotocin diabetes figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected 22. Pdf phytochemicals, hypoglycemic and hypolipidemic. The responses are accompanied by corresponding inverse changes in plasma insulin and sequential ultrastructural. This study evaluated the longterm effects of alloxan induced diabetes in rat liver. Variables in alloxaninduced and fructose induced diabetic rats adesoji a fasanmade and oluwakemi t alabi departments of physiology and medicine college of medicine, university of ibadan ibadan, nigeria abstract honey contains a high concentration of fructose, a monosaccharide, capable of raising blood sugar level after oral ingestion. Data show that treatment with the extract drastically reduced the glucose level. Antidiabetic effect of morinda lucida stem bark extracts on. Palliative effects of daonil supplemented with tryptophan on. Jun 25, 2009 stz and alloxan induced diabetic mice hi im working in animal models mice. Antidiabetic activity of vinca rosea extracts in alloxan.
Wheatgrass has many characteristics desirable for health. Original article modulatory influence of parkia biglobosa. This study describes a rat diabetes mellitus model induced by administering a reduced dose of alloxan, thus greatly reducing the animals death rate. To model diabetes type 2 a low dose of stz may be used in week old rats. Sir, we read with interest the article titled anomalies in alloxan induced diabetic model. Morin treatment attenuated hyperalgesia and analgesia p topiramate, levetiracetam and zonisamide in vincristine induced neuropathic pain in albino rats using chemical method punam jakhar1, omi chouhan2, anusuya gehlot 2, r. Subjects a total of 254 male spraguedawley rats 250300g, obtained from. We thus tested in this work whether a prolonged treatment with tyramine could improve glucose tolerance in streptozotocininduced diabetic rats. Bioactive extracts of moringa reverted glucose level to normal in alloxaninduced diabetic rats. Neurotoxicity induced by a single oral dose of aniline in rats. Anion gap toxicity in alloxan induced type 2 diabetic rats. Mechanisms of inhibited lipolytic response to isoproterenol by metformin in primary rat adipocytes this study is to investigate this study is to investigate the inhibitory effects of metformin on isoproterenol induced lipolysis in primary rat adipocytes and further elucidate the. Moreover, prefrontal mda and sod were not significantly different between all the groups p0. The present study was carried out to investigate the effects of onion allium cepa linn and garlic allium sativum linn juices on biochemical parameters, enzyme activities and lipid peroxidation in alloxaninduced diabetic rats.
Thirty nondiabetic control rats nc and 30 untreated diabetic ud rats were divided into three subgroups sacrificed after 6, 14, or 26 weeks. This was closely associated with a concomitant reduction in levels of stt and reduced testes weight, a noticeable decline in the glutathionestransferase gst. This study was conducted to investigate the effect of nicotinamide on experimentallyinduced iddm. Ameliorative potential of morin in streptozotocininduced. The current study sought to investigate the capacities of single and combinatorial herbal formulations of leaf extracts of acanthus montanus, asystasia gangetica, emilia coccinea, and hibiscus rosasinensis to reverse hyperglycemia and dyslipidemia in alloxaninduced diabetic male rats. Effect of bauhinia holophylla treatment in streptozotocininduced. Alloxaninduced diabetes triggers the development of. Anti diabetic activity of ethanolic extract of aconitum ferox in alloxan induced diaseased rats j kondal reddy1, p sheetal2, rajesh menda2, nukala aparna3 1department of pharmacology, vision college of pharmaceutical sciences, boduppal, hyderabad, india.
Adult male rats 8 weeks, weighing 195 to 225 g was made diabetic by injecting stz 65 mg kg1 body weight intraperitoneally. Antidiabetic activity of date seed methanolic extracts in alloxan induced diabetic rats. Forty male rats were uninephrectomized from the left flank. A comparative study of the effect of gabapentin, topiramate. The results revealed a significant elevation in thiobarbituric acid reactive substances tbars levels in the brain and testes of diabetic rats. Antihyperglycaemic activity of cassia kleinii leaf extract. Enhancement of bilirubin excretion in alloxan induced. First, tyramine content of standard rodent chow was determined by hplc and daily tyramine intake of control rats was estimated to. Bertoni on alloxaninduced dia betic rats bertoni on alloxaninduced dia betic rats himanshu misra, 1, 2 manish soni, 3 narendra silawat, 4 darshana mehta, 1 b. What is the best method to induce type 2 diabetes rat model. A comparative study of the effect of gabapentin, topiramate, levetiracetam and zonisamide for neuropathic pain induced by anticancer drug vincristine in rats punam jakhar1, omi chouhan1, anusuya gehlot1, r. Histopathological and immunohistochemical study of antidiabetic. Differential effect of honey on selected variables in alloxan.
However, in absence of aggressive stimuli such as ligatures, the influence of diabetes on rat periodontal tissues is incompletely explored. Early renal histological changes in alloxaninduced diabetic rats. It is better to standardize it first by jain et al. Alloxan induced diabetic rats were divided into 5 groups of 5 each and were treated with 100, 200 and 400 mgkg of the methanol extract. Hypoglycemic effects in alloxaninduced diabetic rats of the. Early renal histological changes in alloxan induced diabetic rats mohsen pourghasem 1, ebrahim nasiri 2, hamid shafi 3 1. Effect of ginger zingiber officinale extracts on blood glucose in normal and streptozotocininduced diabetic rats. In diabetic rats, the fasting blood glucose level, indexes of heart and liver, and levels of cholesterol and triglyceride in serum and mda in heart. A streptozotocin stzinduced diabetes in animal model mimics, in some aspects, recent onset iddm. The diabetic state induced by streptozotocin injection is known to impair oligodendroglial. Effect of prolonged treatmen with tyramine on glucose. Antidiabetic activity of mediumpolar extract from the leaves of stevia rebaudiana bert.